Poly(d,l-lactide-co-glycolide)–chitosan composite particles for the treatment of lung cancer
نویسندگان
چکیده
Tumor heterogeneity makes combination chemotherapy one of the preferred modes of treatment regimens. In this work, sequential exposure of two anticancer agents, paclitaxel (Tx) followed by topotecan (TPT), was shown to have a synergistic effect on non-small cell lung cancer (NSCLC) cell line, NCI-H460. In order to improve patient compliance, the aforementioned concept was translated into a drug delivery system comprising of poly(d,l-lactide-co-glycolide) (PLGA)-chitosan composite particles. TPT-containing chitosan micro-/nanoparticles were prepared by the facile technique of electrospraying and encapsulated within PLGA microparticles using emulsion-solvent evaporation technique for delayed release of TPT. The formulation containing Tx- and TPT-loaded composite particles demonstrated synergism when exposed to NCI-H460 cellular aggregates (tumoroids) generated in vitro. Overall, the results of this study demonstrated the potential of the formulation containing Tx and PLGA-chitosan (TPT-loaded) composite particles for the treatment of lung cancer.
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*Correspondence: R. M. Mainardes. Departamento de Farmácia. Laboratório de Nanotecnologia Farmacêutica. Universidade Estadual do Centro-Oeste – UNICENTRO. Rua Simeão Camargo Varela de Sá, 03 85040-080 Guarapuava – PR, Brasil. E-mail: [email protected] A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles
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